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azgp1 zap  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology azgp1 zap
    The gene expression profiles were established by comparing the differentially expressed genes (DEGs) of lung tumours from the taurine (25 mg/kg, n = 3), proline (25 mg/kg, n = 3), model ( n = 3) and control ( n = 3) group. In heat-map analysis, the comparison of the top 66 and top 10 regulatory genes of tumours revealed in control vs. model vs. proline ( A ) and control vs. model vs. taurine groups ( B ), respectively. In the histogram, 4 DEGs including Cxcl9, Zbp1, Hmgcs2, and <t>Azgp1</t> were ultimately identified in control vs. model vs. proline groups ( C ). And 4 DEGs including Azgp1, Cd3d, Cd8a and Cyp2d10 were ultimately identified in control vs. model vs. taurine groups ( D ). The criteria of |log 2 FC| ≥ 1 and p < 0.05 was utilised to identify the DEGs with biological significance. Azgp1 was down-regulated in human primary LUSC ( E ) and LUAD ( F ). Azgp1 mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 52) normal human lung tissues available at the TCGA database. Lung tumours with low Azgp1 expression correlated with poor survival ( G ). Statistical significance was calculated by means of one-way ANOVA or t -test analysis, * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Azgp1 Zap, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/azgp1+zap/ZAG+Antibody/pmc11953302-308-21-23
    Average 93 stars, based on 56 article reviews
    azgp1 zap - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway"

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway

    Journal: NPJ Precision Oncology

    doi: 10.1038/s41698-025-00872-2

    The gene expression profiles were established by comparing the differentially expressed genes (DEGs) of lung tumours from the taurine (25 mg/kg, n = 3), proline (25 mg/kg, n = 3), model ( n = 3) and control ( n = 3) group. In heat-map analysis, the comparison of the top 66 and top 10 regulatory genes of tumours revealed in control vs. model vs. proline ( A ) and control vs. model vs. taurine groups ( B ), respectively. In the histogram, 4 DEGs including Cxcl9, Zbp1, Hmgcs2, and Azgp1 were ultimately identified in control vs. model vs. proline groups ( C ). And 4 DEGs including Azgp1, Cd3d, Cd8a and Cyp2d10 were ultimately identified in control vs. model vs. taurine groups ( D ). The criteria of |log 2 FC| ≥ 1 and p < 0.05 was utilised to identify the DEGs with biological significance. Azgp1 was down-regulated in human primary LUSC ( E ) and LUAD ( F ). Azgp1 mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 52) normal human lung tissues available at the TCGA database. Lung tumours with low Azgp1 expression correlated with poor survival ( G ). Statistical significance was calculated by means of one-way ANOVA or t -test analysis, * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: The gene expression profiles were established by comparing the differentially expressed genes (DEGs) of lung tumours from the taurine (25 mg/kg, n = 3), proline (25 mg/kg, n = 3), model ( n = 3) and control ( n = 3) group. In heat-map analysis, the comparison of the top 66 and top 10 regulatory genes of tumours revealed in control vs. model vs. proline ( A ) and control vs. model vs. taurine groups ( B ), respectively. In the histogram, 4 DEGs including Cxcl9, Zbp1, Hmgcs2, and Azgp1 were ultimately identified in control vs. model vs. proline groups ( C ). And 4 DEGs including Azgp1, Cd3d, Cd8a and Cyp2d10 were ultimately identified in control vs. model vs. taurine groups ( D ). The criteria of |log 2 FC| ≥ 1 and p < 0.05 was utilised to identify the DEGs with biological significance. Azgp1 was down-regulated in human primary LUSC ( E ) and LUAD ( F ). Azgp1 mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 52) normal human lung tissues available at the TCGA database. Lung tumours with low Azgp1 expression correlated with poor survival ( G ). Statistical significance was calculated by means of one-way ANOVA or t -test analysis, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Gene Expression, Control, Comparison, Expressing

    Azgp1 is significantly correlated with lipid metabolic pathways in 1017 lung cancer samples (including LUAD and LUSC) according to GSEA ( A – H ). Enrichment scores and p value was calculated in default parameters. |NES| > 1, NOM p val < 0.05, FDR q val < 0.25 were considered to be significant functional pathways. mTOR was up-regulated in human primary LUAD ( I ) and LUSC ( J ). mTOR mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 53) normal human lung tissues available at the TCGA database. mTOR, p-mTOR, Azgp1, and β-actin protein expressions were evaluated by western blot assay ( K , n = 3). The significance of differences was calculated using two-tailed unpaired Student’s t test, * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: Azgp1 is significantly correlated with lipid metabolic pathways in 1017 lung cancer samples (including LUAD and LUSC) according to GSEA ( A – H ). Enrichment scores and p value was calculated in default parameters. |NES| > 1, NOM p val < 0.05, FDR q val < 0.25 were considered to be significant functional pathways. mTOR was up-regulated in human primary LUAD ( I ) and LUSC ( J ). mTOR mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 53) normal human lung tissues available at the TCGA database. mTOR, p-mTOR, Azgp1, and β-actin protein expressions were evaluated by western blot assay ( K , n = 3). The significance of differences was calculated using two-tailed unpaired Student’s t test, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Functional Assay, Expressing, Western Blot, Two Tailed Test

    LLC cells were transduced by lentivirus, and the resistant cells were selected with corresponding antibiotics to obtain mouse Azgp1 overexpression stable cell lines and the control virus transfected cell lines ( A ). In vitro experiments, quantitative RT-PCR was conducted to determine the expressions of Azgp1 in LLC (wt) and LLC (Azgp1 overexpression) cells ( B ). Western blot assay was applied to confirm that gene Azgp1 had been overexpressed ( C ). LLC (wt) and LLC (Azgp1 overexpression) cells viability for 72 h were detected by MTT assays ( D ). The colony formation and statistical analysis of LLC (wt) and LLC (Azgp1 overexpression) cells for 72 h ( E ). Experiments were independently repeated four times. Experiments were independently repeated three times. In vivo experiments, LLC (wt) or LLC (Azgp1 overexpression) cells were injected by subcutaneously into wild type C57BL/6 mice (5 × 10 5 cells per mouse, n = 6 mice per group, F ). Then body weight ( G ) and tumour volumes ( H ) of mice were examined every 3 days. After dissection, the representative images of tumours ( I ) and tumour weights ( J ) were measured and calculated. mTOR, p-mTOR, Azgp1, and β-actin protein expressions in tumours were evaluated by western blot assay ( K , n = 3 mice per group). Statistical significance was calculated by means of ANOVA analysis. All data are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: LLC cells were transduced by lentivirus, and the resistant cells were selected with corresponding antibiotics to obtain mouse Azgp1 overexpression stable cell lines and the control virus transfected cell lines ( A ). In vitro experiments, quantitative RT-PCR was conducted to determine the expressions of Azgp1 in LLC (wt) and LLC (Azgp1 overexpression) cells ( B ). Western blot assay was applied to confirm that gene Azgp1 had been overexpressed ( C ). LLC (wt) and LLC (Azgp1 overexpression) cells viability for 72 h were detected by MTT assays ( D ). The colony formation and statistical analysis of LLC (wt) and LLC (Azgp1 overexpression) cells for 72 h ( E ). Experiments were independently repeated four times. Experiments were independently repeated three times. In vivo experiments, LLC (wt) or LLC (Azgp1 overexpression) cells were injected by subcutaneously into wild type C57BL/6 mice (5 × 10 5 cells per mouse, n = 6 mice per group, F ). Then body weight ( G ) and tumour volumes ( H ) of mice were examined every 3 days. After dissection, the representative images of tumours ( I ) and tumour weights ( J ) were measured and calculated. mTOR, p-mTOR, Azgp1, and β-actin protein expressions in tumours were evaluated by western blot assay ( K , n = 3 mice per group). Statistical significance was calculated by means of ANOVA analysis. All data are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Over Expression, Stable Transfection, Control, Virus, Transfection, In Vitro, Quantitative RT-PCR, Western Blot, In Vivo, Injection, Dissection

    Taurine and proline may inhibit AZGP1 function, triggering mTOR pathway activation and lipid metabolism disorder that ultimately promote lung cancer progression.
    Figure Legend Snippet: Taurine and proline may inhibit AZGP1 function, triggering mTOR pathway activation and lipid metabolism disorder that ultimately promote lung cancer progression.

    Techniques Used: Activation Assay

    Related Articles

    Gene Expression:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Control:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Comparison:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Expressing:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Functional Assay:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Western Blot:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Two Tailed Test:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Over Expression:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Stable Transfection:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Virus:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Transfection:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    In Vitro:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Quantitative RT-PCR:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    In Vivo:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Injection:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Dissection:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Activation Assay:

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: The detailed procedure was reported previously .The detailed procedure was reported previously .. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway.
    Article Snippet: The detailed procedure was reported previously81.The detailed procedure was reported previously81.. The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).. Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuantTM 800Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway
    Article Snippet: Western blot analysis The detailed procedure was reported previously 64.Western blot analysis The detailed procedure was reported previously 64.. The following antibodies were used in this experiment: β-Actin (Cell Signaling Technology (CST), #8457), p-mTOR (Santa cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa cruz, sc-21720).. Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).Amersham ImageQuant™ 800 Western blot imaging systems was used to quantify the signal intensity (Cytiva, Japan).



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    azgp1 zap  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology azgp1 zap
    The gene expression profiles were established by comparing the differentially expressed genes (DEGs) of lung tumours from the taurine (25 mg/kg, n = 3), proline (25 mg/kg, n = 3), model ( n = 3) and control ( n = 3) group. In heat-map analysis, the comparison of the top 66 and top 10 regulatory genes of tumours revealed in control vs. model vs. proline ( A ) and control vs. model vs. taurine groups ( B ), respectively. In the histogram, 4 DEGs including Cxcl9, Zbp1, Hmgcs2, and <t>Azgp1</t> were ultimately identified in control vs. model vs. proline groups ( C ). And 4 DEGs including Azgp1, Cd3d, Cd8a and Cyp2d10 were ultimately identified in control vs. model vs. taurine groups ( D ). The criteria of |log 2 FC| ≥ 1 and p < 0.05 was utilised to identify the DEGs with biological significance. Azgp1 was down-regulated in human primary LUSC ( E ) and LUAD ( F ). Azgp1 mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 52) normal human lung tissues available at the TCGA database. Lung tumours with low Azgp1 expression correlated with poor survival ( G ). Statistical significance was calculated by means of one-way ANOVA or t -test analysis, * p < 0.05, ** p < 0.01, and *** p < 0.001.
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    Image Search Results


    The gene expression profiles were established by comparing the differentially expressed genes (DEGs) of lung tumours from the taurine (25 mg/kg, n = 3), proline (25 mg/kg, n = 3), model ( n = 3) and control ( n = 3) group. In heat-map analysis, the comparison of the top 66 and top 10 regulatory genes of tumours revealed in control vs. model vs. proline ( A ) and control vs. model vs. taurine groups ( B ), respectively. In the histogram, 4 DEGs including Cxcl9, Zbp1, Hmgcs2, and Azgp1 were ultimately identified in control vs. model vs. proline groups ( C ). And 4 DEGs including Azgp1, Cd3d, Cd8a and Cyp2d10 were ultimately identified in control vs. model vs. taurine groups ( D ). The criteria of |log 2 FC| ≥ 1 and p < 0.05 was utilised to identify the DEGs with biological significance. Azgp1 was down-regulated in human primary LUSC ( E ) and LUAD ( F ). Azgp1 mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 52) normal human lung tissues available at the TCGA database. Lung tumours with low Azgp1 expression correlated with poor survival ( G ). Statistical significance was calculated by means of one-way ANOVA or t -test analysis, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: NPJ Precision Oncology

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway

    doi: 10.1038/s41698-025-00872-2

    Figure Lengend Snippet: The gene expression profiles were established by comparing the differentially expressed genes (DEGs) of lung tumours from the taurine (25 mg/kg, n = 3), proline (25 mg/kg, n = 3), model ( n = 3) and control ( n = 3) group. In heat-map analysis, the comparison of the top 66 and top 10 regulatory genes of tumours revealed in control vs. model vs. proline ( A ) and control vs. model vs. taurine groups ( B ), respectively. In the histogram, 4 DEGs including Cxcl9, Zbp1, Hmgcs2, and Azgp1 were ultimately identified in control vs. model vs. proline groups ( C ). And 4 DEGs including Azgp1, Cd3d, Cd8a and Cyp2d10 were ultimately identified in control vs. model vs. taurine groups ( D ). The criteria of |log 2 FC| ≥ 1 and p < 0.05 was utilised to identify the DEGs with biological significance. Azgp1 was down-regulated in human primary LUSC ( E ) and LUAD ( F ). Azgp1 mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 52) normal human lung tissues available at the TCGA database. Lung tumours with low Azgp1 expression correlated with poor survival ( G ). Statistical significance was calculated by means of one-way ANOVA or t -test analysis, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).

    Techniques: Gene Expression, Control, Comparison, Expressing

    Azgp1 is significantly correlated with lipid metabolic pathways in 1017 lung cancer samples (including LUAD and LUSC) according to GSEA ( A – H ). Enrichment scores and p value was calculated in default parameters. |NES| > 1, NOM p val < 0.05, FDR q val < 0.25 were considered to be significant functional pathways. mTOR was up-regulated in human primary LUAD ( I ) and LUSC ( J ). mTOR mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 53) normal human lung tissues available at the TCGA database. mTOR, p-mTOR, Azgp1, and β-actin protein expressions were evaluated by western blot assay ( K , n = 3). The significance of differences was calculated using two-tailed unpaired Student’s t test, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: NPJ Precision Oncology

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway

    doi: 10.1038/s41698-025-00872-2

    Figure Lengend Snippet: Azgp1 is significantly correlated with lipid metabolic pathways in 1017 lung cancer samples (including LUAD and LUSC) according to GSEA ( A – H ). Enrichment scores and p value was calculated in default parameters. |NES| > 1, NOM p val < 0.05, FDR q val < 0.25 were considered to be significant functional pathways. mTOR was up-regulated in human primary LUAD ( I ) and LUSC ( J ). mTOR mRNA expression was compared from 515 human primary LUAD tumours (or 503 human primary LUSC) and 59 (or 53) normal human lung tissues available at the TCGA database. mTOR, p-mTOR, Azgp1, and β-actin protein expressions were evaluated by western blot assay ( K , n = 3). The significance of differences was calculated using two-tailed unpaired Student’s t test, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).

    Techniques: Functional Assay, Expressing, Western Blot, Two Tailed Test

    LLC cells were transduced by lentivirus, and the resistant cells were selected with corresponding antibiotics to obtain mouse Azgp1 overexpression stable cell lines and the control virus transfected cell lines ( A ). In vitro experiments, quantitative RT-PCR was conducted to determine the expressions of Azgp1 in LLC (wt) and LLC (Azgp1 overexpression) cells ( B ). Western blot assay was applied to confirm that gene Azgp1 had been overexpressed ( C ). LLC (wt) and LLC (Azgp1 overexpression) cells viability for 72 h were detected by MTT assays ( D ). The colony formation and statistical analysis of LLC (wt) and LLC (Azgp1 overexpression) cells for 72 h ( E ). Experiments were independently repeated four times. Experiments were independently repeated three times. In vivo experiments, LLC (wt) or LLC (Azgp1 overexpression) cells were injected by subcutaneously into wild type C57BL/6 mice (5 × 10 5 cells per mouse, n = 6 mice per group, F ). Then body weight ( G ) and tumour volumes ( H ) of mice were examined every 3 days. After dissection, the representative images of tumours ( I ) and tumour weights ( J ) were measured and calculated. mTOR, p-mTOR, Azgp1, and β-actin protein expressions in tumours were evaluated by western blot assay ( K , n = 3 mice per group). Statistical significance was calculated by means of ANOVA analysis. All data are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: NPJ Precision Oncology

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway

    doi: 10.1038/s41698-025-00872-2

    Figure Lengend Snippet: LLC cells were transduced by lentivirus, and the resistant cells were selected with corresponding antibiotics to obtain mouse Azgp1 overexpression stable cell lines and the control virus transfected cell lines ( A ). In vitro experiments, quantitative RT-PCR was conducted to determine the expressions of Azgp1 in LLC (wt) and LLC (Azgp1 overexpression) cells ( B ). Western blot assay was applied to confirm that gene Azgp1 had been overexpressed ( C ). LLC (wt) and LLC (Azgp1 overexpression) cells viability for 72 h were detected by MTT assays ( D ). The colony formation and statistical analysis of LLC (wt) and LLC (Azgp1 overexpression) cells for 72 h ( E ). Experiments were independently repeated four times. Experiments were independently repeated three times. In vivo experiments, LLC (wt) or LLC (Azgp1 overexpression) cells were injected by subcutaneously into wild type C57BL/6 mice (5 × 10 5 cells per mouse, n = 6 mice per group, F ). Then body weight ( G ) and tumour volumes ( H ) of mice were examined every 3 days. After dissection, the representative images of tumours ( I ) and tumour weights ( J ) were measured and calculated. mTOR, p-mTOR, Azgp1, and β-actin protein expressions in tumours were evaluated by western blot assay ( K , n = 3 mice per group). Statistical significance was calculated by means of ANOVA analysis. All data are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).

    Techniques: Over Expression, Stable Transfection, Control, Virus, Transfection, In Vitro, Quantitative RT-PCR, Western Blot, In Vivo, Injection, Dissection

    Taurine and proline may inhibit AZGP1 function, triggering mTOR pathway activation and lipid metabolism disorder that ultimately promote lung cancer progression.

    Journal: NPJ Precision Oncology

    Article Title: Taurine and proline promote lung tumour growth by co-regulating Azgp1/mTOR signalling pathway

    doi: 10.1038/s41698-025-00872-2

    Figure Lengend Snippet: Taurine and proline may inhibit AZGP1 function, triggering mTOR pathway activation and lipid metabolism disorder that ultimately promote lung cancer progression.

    Article Snippet: The following antibodies were used in this experiment: β-Actin (Cell Signalling Technology (CST), #8457), p-mTOR (Santa Cruz, sc-293133), mTOR (CST, #2983), Azgp1 (ZAP) (Santa Cruz, sc-21720).

    Techniques: Activation Assay